Biodegradation of low-density polyethylene and polystyrene in superworms, larvae of Zophobas atratus (Coleoptera: Tenebrionidae): Broad and limited extent depolymerization (2023)

As the application of biodegradable polymers has grown, so has the interest in exploring the environmental behaviors of biodegradable microplastics (MPs). In this study, we investigated the interaction of oxytetracycline (OTC) with poly(butylene adipate-co-terephthalate) (PBAT) MPs after biodegradation, and explored the effect of the coexisting Cu(II) on OTC adsorption and desorption processes. The maximum adsorption amounts of virgin PBAT, biofilm PBAT, and degraded PBAT reached 692.05 μg·g⁻¹, 1396.21 μg·g⁻¹, and 1869.93 μg·g⁻¹, respectively, and the presence of Cu(II) increased the OTC adsorption capacities by 431.16 %, 165.99 %, and 132.94 %, respectively. The enhanced adsorption capacities were attributed to the formation of PBAT-Cu-OTC complexes. The remarkable desorption hysteresis of OTC was observed on the degraded PBAT but not on the biofilm PBAT when Cu(II) was present, due to the complexation between Cu(II) and biofilms. The effect of Cu(II) varied depending on the MP physiochemical properties (e.g., surface areas, zeta potentials, and functional groups) and the environmental factors (e.g., the solution pH and coexisting dissolved organic matter). Fourier transform infrared spectroscopy (FTIR) coupled with X-ray photoelectron spectroscopy (XPS) identified the Cu(II) bridging effect, and various interaction forces between PBAT and OTC, including hydrogen-bonding, π-π, cation-π, and electrostatic interactions.

Microplastics(MPs) are ubiquitous, difficult to degrade, and potentially threatening to organisms in marine environment, so it is important to clarify the factors that affect their biogeochemical processes. The impact of biological activities on the MPs in marine environment is ubiquitous and complex, and there is currently a lack of systematic summaries. This paper reviews the effects of biological actions on the migration, distribution and degradation of MPs in marine environment from four aspects: biological ingestion and digestion, biological movement, biological colonization and biological adhesion. MPs in seawater and sediments can be closely combined with organisms through three pathways: biological ingestion, biofilm formation or adhesion to organisms, and are passed between species at different trophic levels through the food chain. The generation and degradation of faecal pellets and biofilms can alter the density of "environmental MPs", thereby affecting their vertical migration and deposition in water bodies. The movement of swimming organisms and the disturbance by benthic organisms can promote the migration of MPs in water and vertical migration and resuspension in sediments, thereby changing the distribution of MPs in local sea areas. The grinding effect of the digestive tract and the secretion of chemicals from the biofilm (such as enzymes and acids) can reduce the particle size and increase surface roughness of MPs, or even degrade them completely. Besides, biological adhesion may be an important mechanism affecting the distribution, migration and preservation of MPs. There may be complex interactions and linkages among marine dynamical processes, photochemical degradation and biological processes that collectively affect the biogeochemical processes of MPs, but their relative contributions remain to be more studied.

Plastics are commonly used in daily life and various industries, generating massive quantities of plastic wastes and causing severe environmental concerns. Thus, the upcycling of plastic wastes into valuable products has recently emerged as an option for addressing the plastic-induced environmental crisis. Since (i) the discovery of plastic-eating species and (ii) the discovery of an enzyme that hydrolyzes polyethylene terephthalate (PET), one of the most commonly used synthetic polymers that is remarkably persistent in the environment, the enzymatic and/or microbial degradation of plastic wastes and the catabolism of plastic monomers have been intensively studied. In this review, we focus on recent advances and key barriers in the biotechnological upcycling of plastic wastes. We also given an overview of the microbial degradation of plastic wastes. Specifically, recent research on the biodegradation of plastics via PET hydrolase (PETase) as a representative plastic-degrading enzyme is discussed. Additionally, this review covers (i) the microbial conversion of plastic monomers from mainly PET and polyurethane (PU) and (ii) the chemobiological upcycling of plastic wastes. Ultimately, this review provides insight into the development of viable biotechnological plastic upcycling systems and contributes to the construction of plastic-based circular carbon economies.

The ever-increasing presence of microplastics in many environmental components has been a cause of worry for humanity due to their small size and potential health risk. Since the last decade, numerous studies have been conducted on the prevalence and dispersion of microplastics. However, at present, there aren't any systematic studies on fate and transport of microplastics that consider multimedia environmental systems and their mitigation measures. Also, there are limited studies on the routes through which humans are exposed to microplastics. In this review, about 380 articles were evaluated to uncover the extent of microplastic fate, transport, and pollution in different environmental components, including soil, freshwater, marine, and atmosphere, as well as its effect on different ecosystems. We gave special attention to understanding many routes and sources of microplastics intended for human consumption and their consequences on human health. Furthermore, we tried to emphasize on the different methods used for sampling, extraction, identification and characterization of microplastics, along with associated benefits and limitations. This study highlighted existing knowledge and gaps in the remediation of microplastics. On this basis, the bottleneck and current challenges have been proposed.

Microplastics, tiny plastic fragments from 1 μm to 5 mm, are widespread globally, even in remote environments. Due to their small sizes, they are easily ingested by organisms and contaminate the food chain. Recently, the biodegradation of some recalcitrant plastics by larva of Tenebrio molitor L. (mealworm) has been reported. However, the effects of microplastic feeding on them are limited. In our study, we selected rigid micro-polystyrene (MPS) as the model plastic to investigate the influences of particle size and larval age on plastic consumption and degradation, and the effects of microplastic feeding on the survival and development of mealworms at different larval ages. The smaller the microplastic fragment was, the more plastics the mealworms consumed, though there was a limit on particle size. Mealworms of three-month-old had the highest consumption rate. Both depolymerization and modification on the functional groups were only observed in frass excreted by three-month old mealworms. Additionally, mealworms cofed with wheat bran and MPS of this age had comparable mortality, larval growing curve and pupation distribution as the control group with wheat bran. Our results demonstrated that mealworms in this larval stage had the greatest resistance to high doses of microplastic feeding. We suggested that microplastic waste could be provided to three-month old mealworms as half replacement of bran diet to result in the greatest plastic consumption and degradation.

Plastic pollution is a major environmental concern due to its deleterious effects on various ecosystems. The limitations and shortcomings of waste management strategies has led to the over-accumulation of plastic waste, mainly comprised of single-use plastics, such as polystyrene (PS). Considering the advantages of biotransformation over the other plastic disposal methods, it has become a major focus of the modern research. Biotransformation of plastics involves its microbial hydrolysis into short chain oligomers and monomers that are eventually assimilated as carbon source by the microbes leading to the release of CO₂. As fungi are known to possess multifarious and highly regulated enzyme system capable of utilizing diverse nutrient sources, the present study explored the potential of Lichtheimia ramosa AJP11 towards myco-transformation of polystyrene sulfonate (PSS), a structural analogue of polystyrene (PS). During the 30-day incubation period of L. ramosa AJP11 in minimal salt medium (MSM)+1% PSS, the fungus showed 41.6% increment in its fresh weight biomass, indicating the utilization of PSS as sole carbon source. Further analysis revealed the generation of various reaction intermediates such as alkanes and fatty acids, crucial for the continuum of fungal metabolic pathways. Moreover, detection of PS oligomers such as cyclohexane and 2,4-DTBP confirmed the myco-transformation of PSS. The extracellular fungal protein profile showed considerable overexpression of a 14.4kDa protein, characterized to be a hydrophobic surface binding (Hsb) protein, which is hypothesized to adsorb onto the PSS to facilitate its transformation. Further, in silico analysis of Hsb protein indicated it to be an amphiphilic α-helical protein with ability to bind styrene sulfonate unit via both hydrogen and hydrophobic interactions, with a binding energy of −5.02kcalmol⁻¹. These findings open new avenues for over expression of Hsb under controlled reactor conditions to accelerate the PS waste disposal.

Environmental Pollution, Volume 256, 2020, Article 113265

Science of The Total Environment, Volume 726, 2020, Article 138564

Polystyrene (PS) has been widely used in various fields, whereas this thermoplastic material is generally considered to be resistant to biodegradation. Tribolium castaneum (Coleoptera: Tenebrionidae), a common pest of stored agricultural products, is a powerful model organism for general insect research. In this study, the larvae of T. castaneum were observed chewing and eating extruded polystyrene foam (XPS). Investigation of the gut microbiome of plastic- and bran-fed T. castaneum larvae showed that Acinetobacter sp. was strongly associated with PS ingestion. Additionally, one bacterial strain capable of PS degradation, was successfully isolated from the gut of these larvae and identified as Acinetobacter sp. AnTc-1 by its 16S rDNA sequence. Gel penetration chromatography (GPC), ¹H nuclear magnetic resonance (¹H NMR) spectroscopy, thermo gravimetric analysis (TGA) and scanning electron microscope (SEM) were employed to characterize the PS degradation. After incubation with AnTc-1 for 60days, the mass weight (12.14%) and molecular weight (13%/25%, weight-average molecular weight (Mw)/number-average molecular weight (Mn)) of PS powder were significantly reduced. The results indicated that the isolated strain of Acinetobacter sp. AnTc-1 has PS-degrading capacity. The isolated strain may play a role in the larval gut for biodegradation of PS and has potential to be applied for petroleum-based plastic degradation study and development of remediation approaches.

Chemosphere, Volume 212, 2018, pp. 262-271

Academics researchers and “citizen scientists” from 22 countries confirmed that yellow mealworms, the larvae of Tenebrio molitor Linnaeus, can survive by eating polystyrene (PS) foam. More detailed assessments of this capability for mealworms were carried out by12 sources: five from the USA, six from China, and one from Northern Ireland. All of these mealworms digested PS foam. PS mass decreased and depolymerization was observed, with appearance of lower molecular weight residuals and functional groups indicative of oxidative transformations in extracts from the frass (insect excrement). An addition of gentamycin (30 mg g⁻¹), a bactericidal antibiotic, inhibited depolymerization, implicating the gut microbiome in the biodegradation process. Microbial community analyses demonstrated significant taxonomic shifts for mealworms fed diets of PS plus bran and PS alone. The results indicate that mealworms from diverse locations eat and metabolize PS and support the hypothesis that this capacity is independent of the geographic origin of the mealworms, and is likely ubiquitous to members of this species.

Chemosphere, Volume 191, 2018, pp. 979-989

Commercial production of polystyrene (PS) -a persistent plastic that is not biodegradable at appreciable rates in most environments-has led to its accumulation as a major contaminant of land, rivers, lakes, and oceans. Recently, however, an environment was identified in which PS is susceptible to rapid biodegradation: the larval gut of Tenebrio molitor Linnaeus (yellow mealworms). In this study, we evaluate PS degradation capabilities of a previously untested strain of T.molitor and assess its survival and PS biodegradation rates for a range of conditions (two simulated food wastes, three temperatures, seven PS waste types). For larvae fed PS alone, the %PS removed in the short (12–15h) residence time of the mealworm gut gradually increased for 2–3 weeks then stabilized at values up to 65%. Thirty two-day survival rates were >85% versus 54% for unfed larvae. For mealworms fed ∼10% w/w PS and ∼90% bran, an agricultural byproduct, rates of PS degradation at 25°C nearly doubled compared to mealworms fed PS alone. Polymer residues in the frass showed evidence of partial depolymerization and oxidation. All of the tested PS wastes degraded, with the less dense foams degrading most rapidly. Mealworms fed bran and PS completed all life cycle stages (larvae, pupae, beetles, egg), and the second generation had favorable PS degradation, opening the door for selective breeding.

Science of The Total Environment, Volume 756, 2021, Article 144087

Polypropylene (PP), a fossil-based polyolefin plastics widely used worldwide, is non-hydrolyzable and resistant to biodegradation as a major source of plastic pollutants in environment. This study focused on feasibility of PP biodegradation in the larvae of two species of darkling beetles (Coleoptera: Tenebrionidae) i.e., yellow mealworms (Tenebrio molitor) and superworms (Zophobas atratus) using PP foam with number-, weight-, and size-average molecular weights (M_n, M_w, and M_z) of 109.8, 356.2, and 765.0kDa, respectively. The tests were conducted in duplicates with respective larvae (300T. molitor and 200 Z. atratus each incubator) at 25°C and 65% humidity for over a 35-day period. The larvae of T. molitor and Z. atratus fed with PP foam as sole diet consumed PP at 1.0±0.4 and 3.1±0.4mg 100 larvae⁻¹days⁻¹, respectively; when fed the PP foam plus wheat bran, the consumption rates were enhanced by 68.11% and 39.70%, respectively. Gel permeation chromatography analyses of the frass of T. molitor and Z. atratus larvae fed PP only indicated that M_w was decreased by 20.4±0.8% and 9.0±0.4%; M_n was increased by 12.1±0.4% and 61.5±2.5%; M_z was decreased by 33.8±1.5% and 32.0±1.1%, indicating limited extent depolymerization. Oxidation and biodegradation of PP was confirmed through analysis of the residual PP in frass. Depression of gut microbes with the antibiotic gentamicin inhibited PP depolymerization in both T. molitor and Z. atratus larvae. High throughput 16S rRNA sequencing revealed that Citrobacter sp. and Enterobacter sp. were associated with PP diets in the gut microbiome of Z. atratus larvae while Kluyvera was predominant in the T. molitor larvae. The results indicated that PP can be biodegraded in both T. molitor and Z. atratus larvae via gut microbe-dependent depolymerization with diversified microbiomes.

Journal of Hazardous Materials, Volume 416, 2021, Article 125803

Polylactic acid (PLA) is biodegraded rapidly under composting or thermophilic temperature but slowly under natural conditions with substantial microplastics generated. In this study, we examined the feasibility of PLA biodegradation and developed a novel approach for PLA waste management using yellow mealworms (Tenebrio molitor larvae) to achieve biodegradation and resource recovery simultaneously. Results confirmed PLA biodegradation in mealworms as sole PLA and PLA-bran mixtures (10%, 20%, 30% and 50% PLA, wt/wt). Feeding PLA-bran mixtures supported the larval development with higher survival rates and lower cannibal rates than feeding PLA only at ambient temperature. The PLA conversion efficiency was 90.9% with 100% PLA diet and was around 81.5–86.9% with PLA-bran mixtures. A peak insect biomass yield was achieved at a PLA ratio of 20%. PLA biodegradation was verified via detection of chemical and thermal modifications. Gut microbial community analysis indicated that intestinal communities shifted with PLA biodegradation, resulting in clusters with OTUs unique to the PLA diet. Based on these findings, we propose a circular approach for PLA waste management via resource recovery of used PLA as the feedstock for insect biomass production, management of mealworm excrement waste as fertilizer, and utilization of agricultural products for PLA production.